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	Provedor de dados ArchiMer (1) BJM (1) Electron. J. Biotechnol. (1) Autor Cheng,Fei (1) Goux, Didier (1) Hou,Lin (1) Ledauphin, Jerome (1) Mallu,Maheswara Reddy (1) Orvain, Francis (1) Peng,Xiaobang (1) Ronda,Srinivasa Reddy (1) Shang,Zhengchun (1) Takahashi, Eri (1) Vemula,Sandeep (1) Woeste,Keith (1) Zhang,Shuoxin (1) Zhao,Peng (1) Mais... Palavra-chave Cell lysis (3) Biofilm (1) Biological activity (1) Cross flow filtration (1) DNA extraction method (1) DNA purity (1) Dowex (1) Fed-batch fermentation (1) Fungal community (1) GCMS analysis (1) Immuno staining (1) Immunostaining (1) Intertidal sediments (1) Navicula jeffreyi (1) Protein (1) Purification (1) Secondary structure (1) Terminal restriction fragment length polymorphism (1) Transmission electron microscopy (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Text (1) Ano 2016 (2) 2009 (1) País Brazil (1) Chile (1) France (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods ArchiMer Takahashi, Eri; Ledauphin, Jerome; Goux, Didier; Orvain, Francis. There is no universal method that can be applied to extract bound extracellular polymeric substances (EPS) from benthic diatoms of intertidal sediments without causing cell lysis. Six extraction methods were tested on a diatom culture of Navicula jeffreyi to establish the best compromise between high yields of carbohydrate extraction and minimum cell lysis. Extraction with distilled water provoked cell lysis (as already known). The five other extraction methods (dowex resin, artificial seawater of half salinity and extractions after pretreatment with gluteraldehyde by three methods: water, dowex water and dowex buffer) did not provoke cell lysis as shown by transmission electronic microscopy. This result was confirmed by the minimum release of internal... Tipo: Text Palavras-chave: Transmission electron microscopy; Protein; Navicula jeffreyi; Intertidal sediments; Immuno staining; Immunostaining; GCMS analysis; Dowex; Cell lysis; Biofilm. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7418.pdf Production, purification and characterization of recombinant human antithrombin III by Saccharomyces cerevisiae Electron. J. Biotechnol. Mallu,Maheswara Reddy; Vemula,Sandeep; Ronda,Srinivasa Reddy. Background: Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance between hemorrhage and coagulation. The present work demonstrated the production, purification and characterization of recombinant human antithrombin (rhAT) from yeast Saccharomyces cerevisiae BY4741 was demonstrated. After expression of rhAT by S. cerevisiae, the biomass and rhAT concentration were analyzed through fed-batch fermentation process. Results: In fed-batch fermentation, the biomass (maximum cell dry weight of 11.2 g/L) and rhAT concentration (312 mg/L) of the expressed rhAT were achieved at 84 h of cultivation time. The maximum cell lysis efficiency (99.89%) was... Tipo: Journal article Palavras-chave: Biological activity; Cell lysis; Cross flow filtration; Fed-batch fermentation; Purification; Secondary structure. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400012 Soil pretreatment and fast cell lysis for direct polymerase chain reaction from forest soils for terminal restriction fragment length polymorphism analysis of fungal communities BJM Cheng,Fei; Hou,Lin; Woeste,Keith; Shang,Zhengchun; Peng,Xiaobang; Zhao,Peng; Zhang,Shuoxin. Abstract Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbiota, five prewashing agents were compared in terms of their efficiency and effectiveness in removing soil contaminants. Residual contaminants were precipitated by adding 0.6 mL of 0.5 M CaCl2. Four cell lysis methods were applied to test their compatibility with the pretreatment (prewashing + Ca2+ flocculation) and to ultimately identify the optimal cell... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell lysis; DNA extraction method; DNA purity; Terminal restriction fragment length polymorphism; Fungal community. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400817 Registros recuperados: 3 Primeira ... 1 ... Última

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